SOLVED: Quiz 4 The annealing temperature of a DNA duplex is given by the formula: Tm 4(# of G-C basepairs) 2( # of A-T basepairs) Calculate the annealing temperature for the following
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SOLVED: Quiz 4 The annealing temperature of a DNA duplex is given by the formula: Tm 4(# of G-C basepairs) 2( # of A-T basepairs) Calculate the annealing temperature for the following
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SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
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SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule" This equation is: Tm = 2(A+T) + 4(G+C) Where A = # of
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SOLVED: The annealing temperature of a DNA duplex is given by the formula: Tm 4(# of G-C basepairs) 2( # of A-T basepairs) Calculate the annealing temperature for the following primers: 5'ATGCCTATAGAATCGTC3'
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Oligonucleotide Melting Temperature
